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Stem cell and Feeder Cell
Feeder Cell / Human Fetal Fibroblast (HFF)
Human Embryonic Stem Cell (hESC)
Induced Pluripotent Stem cell (iPSC)
Tissue engineering
Feeder Layer Cell Actions and Applications
Human Fetal Fibroblast (HFF)

HyperLink Stem cells are biological cells that can differentiate into other types of cells and can divide to produce more of the same type of stem cells. They are found in multicellular organisms.
In mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in various tissues. In adultorganisms, stem cells and progenitor cells act as a repair system for the body, replenishing adult tissues. In a developing embryo, stem cells can differentiate into all the specialized cells—ectoderm, endoderm and mesoderm (see induced pluripotent stem cells)—but also maintain the normal turnover of regenerative organs, such as blood, skin, or intestinal tissues.
https://en.wikipedia.org/wiki/Stem_cell

Treatment
https://en.wikipedia.org/wiki/Stem_cell#Treatment
Diseases and conditions where stem cell treatment is being investigated include:

Diabetes[76] Rheumatoid arthritis[76] Parkinson's disease[76] Alzheimer's disease[76]
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Osteoarthritis[76] Stroke and traumatic brain injury repair[77] Learning disability due to congenital disorder [78] Spinal cord injury repair [79]
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Heart infarction [80] Anti-cancer treatments [81] Baldness reversal[82] Replace missing teeth [83]
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Repair hearing [84] Restore vision [85] and repair damage to the cornea[86] Amyotrophic lateral sclerosis [87] Crohn's disease [88]
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Wound healing [89] Male infertility due to absence of spermatogonial stem cells [90]  
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Research is underway to develop various sources for stem cells, and to apply stem cell treatments for neurodegenerative diseases and conditions, diabetes, heart disease, and other conditions.[91] Research is also underway in generating organoids using stem cells, which would allow for further understanding of human development, organogenesis, and modeling of human diseases.[92]

In more recent years, with the ability of scientists to isolate and culture embryonic stem cells, and with scientists' growing ability to create stem cells using somatic cell nuclear transfer and techniques to create induced pluripotent stem cells, controversy has crept in, both related to abortion politics and to human cloning.

Hepatotoxicity and drug-induced liver injury account for a substantial number of failures of new drugs in development and market withdrawal, highlighting the need for screening assays such as stem cell-derived hepatocyte-like cells, that are capable of detecting toxicity early in the drug development process.[93]

Embryonic stem cell  
https://en.wikipedia.org/wiki/Embryonic_stem_cell

Embryonic stem cells (ES cells or ESCs) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage pre-implantation embryo.[1][2] Human embryos reach the blastocyst stage 4–5 days post fertilization, at which time they consist of 50–150 cells. Isolating the embryoblast, or inner cell mass (ICM) results in destruction of the blastocyst, a process which raises ethical issues, including whether or not embryos at the pre-implantation stage should have the same moral considerations as embryos in the post-implantation stage of development.[3][4] Researchers are currently focusing heavily on the therapeutic potential of embryonic stem cells, with clinical use being the goal for many labs. These cells are being studied to be used as clinical therapies, models of genetic disorders, and cellular/DNA repair. However, adverse effects in the research and clinical processes have also been reported. HyperLink

Induced pluripotent stem cell
https://en.wikipedia.org/wiki/Induced_pluripotent_stem_cell
 

Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cellthat can be generated directly from adult cells. The iPSC technology was pioneered by Shinya Yamanaka’s lab in Kyoto, Japan, who showed in 2006 that the introduction of four specific genes encoding transcription factors could convert adult cells into pluripotent stem cells.[1] He was awarded the 2012 Nobel Prize along with Sir John Gurdon "for the discovery that mature cells can be reprogrammed to become pluripotent." [2]

Pluripotent stem cells hold promise in the field of regenerative medicine.[3] Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease.

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Tissue engineering
https://en.wikipedia.org/wiki/Tissue_engineering
HyperLink Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological tissues. Tissue engineering involves the use of a tissue scaffold for the formation of new viable tissue for a medical purpose. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own.


Feeder Layer Cell Actions and Applications
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4533020/
HyperLink Cultures of growth-arrested feeder cells have been used for years to promote cell proliferation, particularly with low-density inocula. Basically, feeder cells consist in a layer of cells unable to divide, which provides extracellular secretions to help another cell to proliferate. It differs from a coculture system because only one cell type is capable to proliferate. It is known that feeder cells support the growth of target cells by releasing growth factors to the culture media, but this is not the only way that feeder cells promote the growth of target cells.
In this work, we discuss the different mechanisms of action of feeder cells, tackling questions as to why for some cell cultures the presence of feeder cell layers is mandatory, while in some other cases, the growth of target cells can be achieved with just a conditioned medium. Different treatments to avoid feeder cells to proliferate are revised, not only the classical treatments as mitomycin or γ-irradiation but also the not so common treatments as electric pulses or chemical fixation. Regenerative medicine has been gaining importance in recent years as a discipline that moves biomedical technology from the laboratory to the patients. In this context, human stem and pluripotent cells play an important role, but the presence of feeder cells is necessary for these progenitor cells to grow and differentiate. This review addresses recent specific applications, including those associated to the growth of embryonic and induced pluripotent stem cells. In addition, we have also dealt with safety issues, including feeder cell sources, as major factors of concern for clinical applications.

Human Fetal Fibroblast (HFF)

Why human feeder cells ?

Genetic stability
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The ability of feeder-free culture system to maintain genetic stability of hESC (human embryonic stem cells) remains controversial.

http://www.genomic-instability.org/
https://en.wikipedia.org/wiki/Copy-number_variation


Animal pathogen
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hESC lines derived from MEF (mouse embryonic fibroblast) have animal pathogen contamination issue if for future human therapy.
https://en.wikipedia.org/wiki/Pathogen  
GMP-compliant
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No GMP-compliant human feeder cells product are available in stem cell market.
https://en.wikipedia.org/wiki/Good_manufacturing_practice
 

Our solutions :
Human Fetal Fibroblast (HFF) Feeder Cell

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The highest quality Human Feeder Cell of our team partner the Capstone Biotek

Lowest risk
Completely avoid the risk of xenogeneic introductions.
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Compatability
Mitomycin-C treated human fetal fibroblasts (HFF) support human pluripotent cell without introducing a second species to the culture conditions.
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Long-term culture
Capable of maintaining pluripotency of stem cells (iPS/hESC) under long-term culture.
https://www.cell.com/abstract/S0092-8674(14)01566-9
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High quality
Our Human Fetal Fibroblast, HFFs, are tested comprehensively on human embryonic stem cells and iPS cells to ensure consistent and robust performance.
SOX2, OCT4, SSEA4, and TRA-1-60 are pluripotency markers
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Upgradable:
Different grades of feeder cells (Research grade/GMP grade) When the user develops a product from academic research into a clinical human trial, It is also stipulated in the regulations that the level of reagents used must also be increased.
CBI's Research Grade - and Clinical-Grade HFFs are derived from the same cell line, enabling our users for easily switching from Research Grade to Clinical-Grade of HFF along the research phase develpoment while advancing cell therapy products from research into clinical stage.
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Which are human fetal origin, history traceable, GMP/GTP compliance
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Which are human fetal origin, very affordable, could be easily switched to clinical grade

CBI-HFF is the only source on the market that offers two grades of human feeder cells.
 
Quality control:
Lot-to-lot consistency, negative for bacterial/fungal/mycoplasma/human pathogen contamination.
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Save time and money
Ready to use and for a reasonable price you can focus on your research instead of daily routine cell expansion culture.
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Safe handling
Our comprehensive contamination test include sterility, human pathogen, and mycoplasma detection. This protect users and significantly minimizes the contamination in your lab.
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Create long term cooperation with our customers in different product developmental stages.
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