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FaEChERS

The "Fast, Easy, Cheap, Effective, Rugged, and Safe(FaEChERS)"
Sample Extraction Kit & Method for Pesticide Residues Determination

 

How to shorten the time required of chemical determination

for the pesticide residues in agricultural products, have been a global common issues,

among which the extraction of pesticide residues is the most time-consuming step.

 

A rapid chemical extraction technology developed by our cooperative team,

the FaEChERS is a collection of much more faster and more advanced

kit and method for extractions, that can significantly reduce more than 85% time

for the extraction of pesticide residues in fruits and vegetables.


In spite that
the current most widely & globally applied technology

for extraction of pesticide residues in fruits and vegetables is the QuEChERS

("Quick, Easy, Cheap, Effective, Rugged, and Safe"),

the sample extraction steps and time of our FaEChERS technology is significantly reduced to only 1/30
of QuEChERS  extraction time, that effectively enhances
the timeliness of pesticide residue testing.

Therefore, FaEChERS technology has the very high potential to replace the QuEChERS.

 

We hope to be able to draw on technical and marketing capacity of international companies,
for quick and effective international promotions, including the progressive striving to become the standard methods
in the AOAC, EU CFDA, etc.
, and to will maximize application values and provide important helps

to the international agricultural and analytical fields.

 

Comparison of the extraction steps and time required for FaEChERS and QuEChERS :

QuEChERS

FaEChERS

1. the solvent is added to the sample.

2. add extraction powder with short shaking.

3. drastic shaking

4. centrifuge

5. retrieve the supernatant

1. Add the solvent to the sample with brief shaking and filtration.

6. Add purification powder

7. drastic shaking once again

8. centrifuged again

9. supernatant
10. add to volume

10 total steps, 30 minutes

One step, 1 minute.

 

FaEChERS patents has been filed:

2014, ROC patent

2014, People's Republic of China patent

2014, US patent

More patents pending, ....

 

 

FaEChERS technical features:

1. One step to complete the extraction of pesticide residues in sample.

2. Save more than 85% extraction time required for pesticide residues.

3. Improve the efficiency of pesticide residues extraction about five times.

4. Reduce solvent use by about 50%.

5. Save 60% labor costs for the pesticide residue determinations.

 

Comparison of the performance of extraction kit

Item

Traditional SPE

QuEChERS

FaEChERS

Steps required

<= 20 steps

>= 10 steps

1-2 steps

Time required

60 minutes to 48 hours

30 minutes

1 minutes

Accuracy (recovery)

60% -140%

60% -140%

60% -140%

Precision (coefficient of variation)

< 20

< 20

< 20

Limit of detection (LOQ) <= 0.01ppm

251 pesticides,

310 pesticides

355-408 pesticides (depending on crops)

Linearity (correlation coefficient)

> 0.99

> 0.99

> 0.99

Matrix Effects Assessment

High matrix effects

 

Lower matrix effects

Much lower matrix effects

 

The validation of FaEChERS extraction kits

Recovery(%)

mango

shallots

greens

apples

carrots

avocado

pea sprouts

<60

57

63

61

53

40

42

35

60-140

355

351

353

363

371

401

408

> 140

6

4

6

4

9

1

1

Doses

418

418

420

420

420

444

444

Mango, shallots, greens, apples, carrots, avocado, pea sprouts matrix used:

In the 60% -140% recovery range, there are 355, 351, 353, 363,

371, 401 and 408 items, that respectively account for 85%, 84%, 86%, 88%, 90% and 92% of the total number of pesticides.

The range collectively account up to 94% and 98.6% of the total number

of pesticides that are ever and frequently detected respectively.

The pesticides items with the Limit of detection(LOQ) available to 0.01ppm,

for Mango matched with reagents and methods of FaEChERS, for example,

is up to 388 items, accounting for 92.8% of the total pesticides analyzed.

LOQ(ppm) range(<=)

Items

<=0.01

388

>0.01 <=0.025

15

>0.025 <=0.05

8

>0.05 <=0.1

2

>0.1 <=0.25

4

>0.25 <=0.5

0

>0.5, <=1

1

 

 

 

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