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Bioprocess Services
Gene recombinant technology / Recombinant protein expression
Protein Purification / Analytical Methods Development
Cell-based potency assays / Virus cultivation

Transport of energy and mass is fundamental to many biological and environmental processes. Areas, from food processing to thermal design of building to biomedical devices to pollution control and global warming, require knowledge of how energy and mass can be transported through materials (mass, momentum, heat transfer).
https://en.wikipedia.org/wiki/Bioprocess
                                                    
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Upstream bioprocessing
Therapeutic cell manufacturing processes can be separated into upstream processes and downstream processes. The upstream process is defined as the entire process from early cell isolation and cultivation, to cell banking and culture expansion of the cells until final harvest (termination of the culture and collection of the live cell batch).

Aside from technology challenges, concerning the scalability of culture apparatus, a number of raw material supply risks have emerged in recent years[when?], including the availability of GMP grade fetal bovine serum[citation needed].
The upstream part of a bioprocess refers to the first step in which microbes/cells are grown, e.g. bacterial or mammalian cell lines (see cell culture), in bioreactors.

HyperLink Upstream processing involves all the steps related to inoculum development, media development, improvement of inoculum by genetic engineering process, optimization of growth kinetics so that product development can improve tremendously. Fermentation has two parts: upstream and downstream. After product development, the next step is the purification of product for desired quality. When they reach the desired density (for batch and fed-batch cultures) they are harvested and moved to the downstream section of the bioprocess.
 
Downstream bioprocessing
The downstream part of a bioprocess refers to the part where the cell mass from the upstream are processed to meet purity and quality requirements. Downstream processing is usually divided into three main sections: cell disruption, a purification section and a polishing section. The volatile products can be separated by distillation of the harvested culture without pre-treatment. Distillation is done at reduced pressure at continuous stills. At reduced pressure distillation of product directly from fermentor may be possible. The steps of downstream processing are:
  1. Separation of biomass: separating the biomass (microbial cells) generally carried out by centrifugation or ultra-centrifugation. If the product is biomass, then it is recovered for processing and spent medium is discarded. If the product is extra cellular the biomass will be discarded. Ultra filtration is an alternative to the centrifugation.
  2. Cell disruption: If the desired product is intra cellular the cell biomass can be disrupted so that the product should be released. The solid-liquid is separated by centrifugation or filtration and cell debris is discarded.
  3. Concentration of broth: The spent medium is concentrated if the product is extracellular.
  4. Initial purification of metabolites: According to the physico-chemical nature of the product molecule several methods for recovery of product from the clarified fermented broth were used (precipitation, etc.)
  5. De-watering: If low amount of product is found in very large volume of spent medium, the volume is reduced by removing water to concentrate the product. It is done by vacuum drying or reverse osmosis.
  6. Polishing of metabolites: this is the final step of making the product 98 to 100% pure. The purified product is mixed with several inert ingredients called excipients. The formulated product is packed and sent to the market for the consumers.
 
Based on our very high quality and innovative environments both in public and private domains in our Biotechnology & Pharmaceutical Community,
http://www.chromnet.net/Bio-Community.aspx
We have key professional core technology, abundant practical experiences, extensive resources and integrated capabilities for Bioprocess Services. Our prominent team partners, including the Blue Blood Biotech, have built  Gene recombinant, Recombinant protein expression, Protein Purification, and Analytical Methods Development core technologies, providing efficient service capabilities including Protein Engineering, Protein Expression Development, Recovery & Purification Development, Cell-based Bioassays, Analytical Methods Development, etc.
   
Core Technology  
   
Gene recombinant technology Polymerase chain reaction
DNA electrophoresis
Stable clone screening and selection
   
 
Recombinant protein expression Yeast expression system
Mammalian expression system
 
Protein Purification Lab scale system
Pilot scale system
Production scale system
 
Analytical Methods Development ELISA assays
HPLC-IEX, SEC, RP columns
SDS-PAGE analysis
   
Cell-based potency assays Cell proliferation
Cell migration
‧ Boyden chamber migration
‧ Transwell migration
‧ FluoroBlok migration
‧ Scratch migration
Tube formation
   
Virus cultivation Plaque assay
Neutralization assay
 
Bioprocess Services  1.Protein Engineering
2.Protein Expression Development
3.Recovery & Purification Development
4.Cell-based Bioassays
5.Analytical Methods Development
 
Protein Engineering We can take cell line to develop your project from DNA to clone screening and selection . We have experience working with different types of expression system Ex. Pichia pastoris, CHO, .....
Our service includes :
-Transfection
-Amplification
-Clone screening
-Clone selection
 
Protein Expression Development We provide the upstream culture process development and runs batch bioreactor systems with different type of expression system. We can develop process through a combination of screening, media screening , yield analysis, and systematic optimization of parameters. Key equipment includes 5L New BrunSwick Scientific bioreactors with mass flow controllers for gas mixing, continuous centrifuge, BelloStage-3000, CO2 incubator and Laminar flow.
Service includes :
-Cell propagation in flasks, BelloStage-3000, and Bioreactors
-Clone selection based on productivity/stability
-Media screening/optimization
-Adaptation of cell lines
-Bioreactor parameter optimization
-Development of feed strategies
-Process scale-up Scale-up/Technology transfer
 
 
Recovery & Purification Development Our purification development laboratory is equipped to develop new purification processes, improve and trouble-shoot unit operation of purification process, and systematically evaluate process parameters to improve overall process economics. Key process equipment includes a AKTA purifier10, AKTA Primeplus and fraction collector, AKTA Piolt、Bioprocess system、Lab TFF system、XK &BPG Column、 UniFlux 10A &30A.
Service includes :
-Develop new  recovery/purification processes
-Improve and trouble-shoot existing unit operations
-Column chromatography
-- affinity, HIC, IEX, SEC -TFF, Ultrafiltration
-Scale-up/Technology transfer
 
 
Cell-based Bioassays Cells were purchased from Bioresource Collection and Research Center (BCRC) and American Type Culture Collection (ATCC).
Key equipment includes Pressurized Clean Room, Optima/NIKON microscopies with digital camera, Laminar flow, CO2 incubator, Fluorescent activity assays
Service includes :
-Cell Proliferation
-Cell Migration
-Boyden chamber
-Transwell
-FluoroBlok
-Scratch wound healing
-Tube Formation
 
Analytical Methods Development Services and methods include testing for product/process related impurities anddeveloping release and in-process assays in support of microbial fermentation,cell culture and purification development.
Service includes :
-BCA /Bradford protein assays
-A280 protein determination
-pH
-Conductivity
-ELISA assays
-HPLC-affinity, IEX, HIC, SEC, RP-HPLC
-SDS-PAGE analysis
-IEF analysis
-Western blot analysis
-LAL Endotoxin assays
-Free-thiol assay
-Protease activity assay
-Cellulase activity assay
 

 

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